![]() ![]() They are a hardy, semi-adherent, low-maintenance cell line and divide rapidly, doubling about every 36 hours. There are many advantages of using HEK293 cells. The ‘T’ in the name of this daughter cell line comes from the incorporation of the SV40 large T antigen into the HEK293 genome – this means they are able to produce large amounts of protein from plasmids vectors carrying the SV40 origin of replication. So, are HEK293T cells the same thing? Not exactly. Incorporating the adenoviral genes into the HEK cell genome resulted in the cells becoming very efficient at producing high amounts of recombinant proteins from plasmid vectors carrying the CMV promoter region. It was his 293rd experiment, which is why they got the tag HEK293. They were transfected with sheared adenovirus 5 (Ad5) DNA by Frank Graham, a postdoc in Van der Eb’s lab. HEK293 cells are Human Embryonic Kidney cells, originally isolated and grown by Dutch biologist Alex Van der Eb in the early 1970s. Read on for answers to these questions and more. But what are they? Why should you use them? And what does the ‘293’ mean? Are HEK293T cells the same thing? HEK293 cells are one of the most commonly used cell lines in laboratories across the globe. ![]() If you’re at all familiar with cell culture, you’ve probably heard of HEK293 cells. ![]()
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