![]() ![]() They are a hardy, semi-adherent, low-maintenance cell line and divide rapidly, doubling about every 36 hours. There are many advantages of using HEK293 cells. The ‘T’ in the name of this daughter cell line comes from the incorporation of the SV40 large T antigen into the HEK293 genome – this means they are able to produce large amounts of protein from plasmids vectors carrying the SV40 origin of replication. So, are HEK293T cells the same thing? Not exactly. Incorporating the adenoviral genes into the HEK cell genome resulted in the cells becoming very efficient at producing high amounts of recombinant proteins from plasmid vectors carrying the CMV promoter region. It was his 293rd experiment, which is why they got the tag HEK293. They were transfected with sheared adenovirus 5 (Ad5) DNA by Frank Graham, a postdoc in Van der Eb’s lab. HEK293 cells are Human Embryonic Kidney cells, originally isolated and grown by Dutch biologist Alex Van der Eb in the early 1970s. Read on for answers to these questions and more. But what are they? Why should you use them? And what does the ‘293’ mean? Are HEK293T cells the same thing? HEK293 cells are one of the most commonly used cell lines in laboratories across the globe. ![]() If you’re at all familiar with cell culture, you’ve probably heard of HEK293 cells. ![]()
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He later disguises himself as the Rag Wizard, and dopes Finn and Jake into helping him obtain the memory of Marceline dumping him in the pretense that she put herself in a sleeping spell, and would remain asleep for eternity if they didn't get the memory. The reason for the break up was because he sold her teddy bear Hambo to a witch so he could get a new Cherry Blossom Wand. ![]() ![]() To help you do that, here's a guide on how to find videos on Facebook. Now that you can save Facebook videos, you might want to go back and search for old videos you liked. Why should you use FDownloader FDownloader.Net is a. There are lots of other sites that work the same as FDown. Facebook video download application for Android devices: Download Facebook videos quickly with HD quality. 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For example, showing off a cool stunt, introducing a new mod you've developed, etc.įor posts, if not obvious, you should submit the link as a self-post and include an explanation. Submissions with a specific purpose are not considered self-promotion. This includes but is not limited to YouTube content, blogs/articles, referral links, etc. Be respectful, or you may be banned.ĭo not link or discuss pirated mods, games, or piracy websites. Toxic and aggressive behavior will not be tolerated.ĭo not be rude to any other user. ![]() Keep discussions civil, mature and friendly. I see no easy way to get inside this court yard area. I'm a bit stumped on how to get to this one inside the long red 'barn'. No offensive behavior or inappropriate content. Great the have a goal in most of the maps search and destroy. 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